Microscope Demonstration

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Our research team had an opportunity to see a demonstration of an Olympus two-photon microscope .
This impressive piece of hardware can take focused depth images into a sample.These types of scopes are used specifically with biological samples that canbe fluorescently stained. This is not a simple field microscope; the entiresystem was mounted on a large stabilized table with a fairly powerful laserand the sensitivity of the microscope required the room to be in completedarkness.Much of the presentation was outside my area of expertise. However, ofparticular interest to me was the ease at which the technician was able togenerate beautiful, high resolution images using multiple modalities. It would be fun to have a project that would allow us to play with one so Icould learn more about the technology and how it works. I hope the EngineeringSummer Undergraduate Research Experience(EnSURE) students found the demonstration motivating and not too technical.

The following is an image generated with the microscope of a rabbit tongue:
Some additional info from the demonstration technician: "Red is fluorescence staining with Eosin. Green and Cyan are second harmonicgeneration imaging of collagen in the forward and reflected directions,respectively (and simultaneously with fluorescence). Excitation wavelength was 860nm and the emission was in the violet channels (430nm for SHG) (forward and reflected) and red (reflected)."

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